Effect of glucosamine chondroitine sulphate on repaired tenotomized rat Achilles tendons
Hamza Özer1, Anıl Taşkesen1, Oğuz Kul2, Hakan Y. Selek1, Sacit Turanlı1, Kenan Köse3
1Gazi Üniversitesi Tıp Fakültesi Ortopedi ve Travmatoloji Anabilim Dalı, Ankara, Türkiye;
2Kırıkkale Üniversitesi Veteriner Fakültesi, Patoloji Anabilim Dalı, Kırıkkale, Türkiye;
3Ankara Üniversitesi Tıp Fakültesi Biyoistatistik Anabilim Dalı, Ankara, Türkiye;
Keywords: Achilles tendon; inflammation; glucoseamine; primary repair.
Abstract
Objectives: We evaluated the effects of glucosamine chondroitine sulphate (glcN-CS) on rat Achilles tendons in this experimental animal study. Materials and methods: Thirty Wistar albino type rats weighing 300-350 g were randomly grouped into two. Group A: Achilles tendons were tenotomized, repaired and, glcN-CS given orally. Group B: Achilles tendons were tenotomized and repaired. All the rats were fed with pellet chow. Five rats in each group were sacrificed at 4, 8, and 12 weeks interval. Tensile strength of three tendon specimen from each group were tested biomechanically and two specimen were analysed histopathologically via immunohistochemical staining and hematoxylin and eosin staining. Contralateral side Achilles tendons were also grouped and analyzed as controls (group C and D).
Results: Histological results showed well organized collagen formation and less inflammation in group A. Biomechanical testing showed higher tendon strength in group A at eight weeks which is not statisticaly significant (55.3±4.84N, p=0.078). Rats which were were given glcN-CS had greater tendon strength which is statisticaly significant (50.01±5.62, p=0.014).
Conclusion: Glucoseamine chondroitine sulphate improved results of Achilles tendon healing in rats. This result might be due to decreased inflammation and stimulation of collagen synthesis. Although glcN-CS is recommended only in osteoarthritic patients to relieve symptoms and signs it can also be prefered in treatment of soft tissue injuries formed during sports activities.