EFFECTS OF THE CALCITONIN HORMONE ON FRACTURE HEALING
Ercan ÇETİNUS1, Metin AKGÜMÜŞ2, İlhan CEVER3, Ö Faruk ATAY4, Sevgi BAKARİŞ5
1Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Ortopedi ve Travmatoloji Anabilim Dalı
2SB Adapazarı Devlet Hastanesi Ortopedi ve Travmatoloji Kliniği
3SB Haseki Hastanesi Ortopedi ve Travmatoloji Kliniği
4SB Haseki Hastanesi Patoloji Kliniği
5Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı
Keywords: Fracture Healing, Calcitonin, Callus.
Objective: The goal of this study is to investigate the role of systemic calcitonin (Salmon calcitonin) application on the healing of fractures in a rat model at the light microscopic level. Material and
Method: 40 adult wistar albino rats were divided into two groups as control (K) and calcitonin (C) randomly. Surgical fractures to the diaphysis of tibia and fibula were applied to all rats under sterile conditions and ketamin anesthesia. Fractures were fixed by intramedullary application of the metal part of injector needle. Salmon calcitonin (Miacalcic Sandoz) was given to the calcitonin group subcutaneously at a dose of 10 MRC-U/kg/day for 20 days after the operation. No treatment was given to the control group. Rats, divided into quadruplicate groups in a randomized manner previously, were sacrificed in the 5th, 12th, 19th, 26th and 56th days postoperatively and fractured tibiae were evaluated histologically.
Results: In the histological evaluation of the site of fracture, hematoma formation in the 5th day, fibrous callus tissue in the 12th day, and cartilagineous callus tissue in the 19th day were observed in both groups. Osteoid tissue formation was seen in the fracture site in both groups in the 26th day after the operation. In the postoperative 56th day, nonlamellar bony callus formation which cannot be cut by regular surgical blade macroscopically and requiring decalsification in both groups were observed. Conclusion : When all the steps of fracture healing were revealed, no significant difference was found in any of those steps among the calcitonin and control groups histologically.