Interaction of MC3T3-E1 cells with titanium implants
Petek Korkusuz1, Sema S. Hakkı2, Nuhan Puralı3, İlker Görür4, Ercüment Önder5, Rahime Nohutçu6, Nursen Koç7, Muharrem Timucin7, Adnan Öztürk8, Feza Korkusuz9
02Selçuk University Faculty of Dentistry, Department of Periodontology, Konya
3. Hacettepe University, Faculty of Medicine, Department of Biopyhsics, Ankara
1Hacettepe University, Faculty of Medicine, Department of Histology and Embryology, Ankara
3Hacettepe University, Faculty of Medicine, Department of Biopyhsics, Ankara
4Ankara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Ankara
5Middle East Technical University, Medical Center, Ankara, Turkey
6Hacettepe University, Faculty of Dentistry, Department of Periodontology, Ankara, Turkey
7Middle East Technical University, Faculty of Engineering, Department of Metallurgical and Materials Engineering, Turkey
8Ankara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Ankara, Turkey
9Middle East Technical University, Department of Biomedical Engineering and Medical Center Ankara, Turkey
Keywords: Titanium, Implant, Bone, Osteoblast cell culture, MC3T3-E1, Scanning electron microscopy, Confocal microscopy.
Objectives: The aim of the present study was to evaluate in-vitro MC3T3- E1 preosteoblastic cell osseointegration on surfaces of polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Materials and methods: MC3T3-E1 cell proliferation and mineralization was assessed comparatively on polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Cell morphology, attachment and proliferation were also comparatively evaluated using confocal (CM) and scanning electron microscopy (SEM).
Results: All implants used in this study were biocompatible. Cells started to attach on the surfaces of the implants following exposure to the in vitro medium for 3 days. The cells were viable and metabolically active as observed by CM. Cell population increased exponentially from day 3 to day 22. Proliferation rate was highest on polished surfaces and lowest on sodium titanate-coated surfaces. In contrast, mineralized nodules were numerous on sand-blasted and sodium titanatecoated surfaces when compared to the polished ones on day 30.
Conclusion: This study demonstrated that sand-blasting and sodium titanate coating provided by NaOH favored the attachment, mineralization and early differentiation of osteoblasts on titanium alloys.